The Use of Hydrocyclones for Mammalian Cell Retention
A new type of hydrocyclone was designed and used in continuous mammalian cell cultivation processes The apparatus gave cell separation efficiencies as high as 99% Due to the fact that a 5Lbioreactor was used, a suboptimal configuration of overflow and underflow diameters was used in order to obtain high cell viabilities combined with06/09/2006· A hydrocyclone with a volume of 256 cm3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume) For the feasible operation range (09 toUse of Hydrocyclones for Mammalian Cell Retention:
Use of Hydrocyclones for Mammalian Cell Retention:
06/09/2006· A hydrocyclone with a volume of 256 cm 3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume) For the feasible operation range (09 to 16 L/min flow corresponding to pressure drops of 04 to 13 bar) the hydrocyclone was characterized with regard to flow split (underflowtooverflow ratio) and flow ratio (underflow toThe results indicate that the hydrocyclone can be appropriately used for cell retention and separation in perfusion cultures A hydrocyclone with a volume of 256 cm3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume) For the feasible operation range (09 to 16 L/min flow corresponding to pressure drops of 04 to 13 bar) theUse of Hydrocyclones for Mammalian Cell Retention: Separation
Hydrocyclones as cell retention device for CHO perfusion
Abstract In this study, a hydrocyclone (HC) especially designed for mammalian cell separation was applied for the separation of Chinese hamster ovary cells The effect of key features on the separation efficiency, such as type of pumphead in the peristaltic feed pump, use of an auxiliary pump to control the perfusate flow rate, and tubing size14/11/2007· The best performance was obtained with a 10mm DorrOliver hydrocyclone, resulting in a separation efficiency of 81% These experimental values agree with computational fluid dynamics (CFD) predictions that high levels of separation efficiencies for mammalian cells could be achieved with smalldiameter hydrocyclones (Medronho et al 2005)Separation of CHO cells using hydrocyclones
Effects of hydrocyclones on the integrity of animal and
The comparative effects of passing mammalian and nucleated red blood cells through liquid/liquid (low shear) and solid/liquid (high shear) hydrocyclones have been examined Cell disruption was only found when the rig was operated in conjunction with a solid/liquid hydrocyclone apparently as a result of the higher operating pressures requiredAbstract In this study, a hydrocyclone (HC) especially designed for mammalian cell separation was applied for the separation of Chinese hamster ovary cells The effect of key features on the separaSkip to Article Content; Skip to Article Information; Search within Search term Advanced Search Citation Search Search term Advanced Search Citation Search Login /Hydrocyclones as cell retention device for CHO perfusion
Separation of CHO cells using hydrocyclones PubMed
When cells were monitored for any induction of apoptosis upon passage through the hydrocyclones, no increase in apoptotic cell concentration was observed within 48 h of hydrocycloning Thus, based on the high separation efficiencies, the robustness of the equipment, and the absence of apoptosis induction, hydrocyclones seem to be specially suited for use14/11/2007· High cell separation efficiencies, varying from 979% to 999%, were obtained for 5 out of 6 hydrocyclone configurations The geometry 3010 (D u = 30 mm and D o = 10 mm) presented a flow ratio of 100%, diverting the total feed flow to the underflow, indicating that this configuration is inappropriate to perform any kind of cell separation Loss of viability wasSeparation of CHO cells using hydrocyclones | SpringerLink
The Use of Hydrocyclones for Mammalian Cell Retention in
separation efficiencies of 81 % for one hydrocyclone and 94% for two in series could be obtained, even employing commercial hydrocyclones that were not specifically designed for application in mammalian cell cultivation (Liibberstedt et aI, 2000a, b; Deckwer et a!, 2000) Moreover, no changes in viability were observed when the hydrocyclones wereAbstract In this study, a hydrocyclone (HC) especially designed for mammalian cell separation was applied for the separation of Chinese hamster ovary cells The effect of key features on the separation efficiency, such as type of pumphead in the peristaltic feed pump, use of an auxiliary pump to control the perfusate flow rate, and tubing sizeHydrocyclones as cell retention device for CHO perfusion
application of hydrocyclone for cell separation in mammalian cell
The present work was carried out to investigate the possibility of hydrocyclone application for cell separation in mammalian cell perfusion cultures Firstly, experiments were carried out to evaluate the physical characteristics controlling the separation performance of the hydrocyclone as well as the effect of the different operating conditions and different hydrocyclone14/11/2007· The best performance was obtained with a 10mm DorrOliver hydrocyclone, resulting in a separation efficiency of 81% These experimental values agree with computational fluid dynamics (CFD) predictions that high levels of separation efficiencies for mammalian cells could be achieved with smalldiameter hydrocyclones (Medronho et al 2005)Separation of CHO cells using hydrocyclones
Application of hydrocyclones for continuous cultivation of SP2/0 cells
22/11/2011· Hydrocyclones (HCs) have been recently extensively evaluated for their application in the separation of mammalian cells in perfusion bioreactors The high centrifugal force derived within hydrocyclones is the key mechanism for cell separation inside such smallsized simple devices This is usually accompanied by a very short residence time ofApplication of Hydrocyclone for Cell Separation in Mammalian Cell Perfusion Cultures Ebook written by Elsayed Ahmed Elsayed Ahmed Read this book using Google Play Books app on your PC, android, iOS devices Download for offline reading, highlight, bookmark or take notes while you read Application of Hydrocyclone for Cell Separation in Mammalian Cell PerfusionApplication of Hydrocyclone for Cell Separation in Mammalian Cell
Data Hydrocyclone SBL2001e Hydrocyclone Mammalian cell
hydrocyclone mammalian cell retention device for and reusable bioreactors perfusion technologies overflow (harvest) feed feed product specification material Anmelden Registrieren Anmelden RegistrierenApplication of Hydrocyclone for Cell Separation in Mammalian Cell Perfusion Cultures Vom Fachbereich für Biowissenschaften und Psychologie der Technischen Universität CaroloWilhelmina zu Braunschweig zur Erlangung des Grades eines Doktors der Naturwissenschaften (Dr rer nat) genehmigte Dissertation von Elsayed Ahmed Elsayed Ahmed aus Kairo,Application of Hydrocyclone for Cell Separation in Mammalian Cell
Acoustic Settler, Centrifuge, Hydrocyclone Animal
Hydrocyclone A detailed description of the hydrocyclone for mammalian cell culture can be found in Castilho's review (Castilho and Medronho 2002) Hydrocyclones are often used to separate solids from liquids or liquids withAlthough DNA breakage and reunion in nonhomologous recombination are poorly understood, previous work suggests that short sequence homologies may play a role in the endjoining step in mammalian cells To study the mechanism of end joining in more detail, we inserted a polylinker into the simian virus 40 Tantigen intron, cleaved the polylinker with different pairs of restrictionNonhomologous recombination in mammalian cells: role for
(PDF) The Potential of Hydrocyclone Application for Mammalian Cell
The Potential of Hydrocyclone Application for Mammalian Cell Separation in Perfusion Cultivation Bioreactors International Journal of Biotechnology for Wellness Industries, 2013The comparative effects of passing mammalian and nucleated red blood cells through liquid/liquid (low shear) and solid/liquid (high shear) hydrocyclones have been examined Cell disruption was only found when the rig was operated in conjunction with a solid/liquid hydrocyclone apparently as a result of the higher operating pressures requiredEffects of hydrocyclones on the integrity of animal and microbial cells
Application of hydrocyclones for continuous cultivation of SP2/0 cells
22/11/2011· Hydrocyclones (HCs) have been recently extensively evaluated for their application in the separation of mammalian cells in perfusion bioreactors The high centrifugal force derived within hydrocyclones is the key mechanism for cell separation inside such smallsized simple devices This is usually accompanied by a very short residence time ofThe present work was carried out to investigate the possibility of hydrocyclone application for cell separation in mammalian cell perfusion cultures Firstly, experiments were carried out to evaluate the physical characteristics controlling the separation performance of the hydrocyclone as well as the effect of the different operating conditions and different hydrocycloneApplication of Hydrocyclone for Cell Separation in Mammalian Cell
hydrocyclone mammalian cells
Use of Hydrocyclones for Mammalian Cell Retention · A hydrocyclone with a volume of 256 cm3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume) For the feasible operation range (09 to 16 L/min flow corresponding to pressure drops of 04 to 13 bar) the hydrocyclone was characterized with regard to flow splitHighly Efficient Extraction of Aromatic Compounds from Houttuyniacordata thumb by Cryogenic Grinding TechniquesAbstract : The Potential of Hydrocyclone Application for Mammalian Cell
A twostep microengineered system for highdensity cell
01/01/2021· The use of hydrocyclones for mammalian cell separation has previously been shown as well , , , However, those studies were limited to low cell densities (<10 million cells/mL) [9] , [45] Few propose that further development of these hydrocyclones, particularly if optimized to specifically suit mammalian cells, could make them competitive withFor the first time, a hydrocyclone specially designed for mammalian cell separation is reported to enable perfusion processes at cell densities in the range of 20 to 50 million/mL for 20 to 25 days Also for the first time, a HC is connected to a singleuse bioreactorHydrocyclone for mAb production in a perfusion singleuse
A Review on Centrifugation in the Pharmaceutical Industry
02/05/2015· Nycodenz®, Histodenz™ Mammalian cells, organelles, membrane vesicles, viruses Iodixanol Mammalian cells, organelles, membrane vesicles, viruses, plasma lipoproteins, proteins, DNA Colloidal silica media Percoll® Mammalian cells, organelles, membrane vesicles (infrequent) 5 Classification of Industrial Centrifuges A Perforated bowl or filter types 1Although DNA breakage and reunion in nonhomologous recombination are poorly understood, previous work suggests that short sequence homologies may play a role in the endjoining step in mammalian cells To study the mechanism of end joining in more detail, we inserted a polylinker into the simian virus 40 Tantigen intron, cleaved the polylinker with different pairs of restrictionNonhomologous recombination in mammalian cells: role for
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The Use of Hydrocyclones for Mammalian Cell Retention
A new type of hydrocyclone was designed and used in continuous mammalian cell cultivation processes The apparatus gave cell separation efficiencies as high as 99% Due to the fact that a 5Lbioreactor was used, a suboptimal configuration of overflow and underflow diameters was used in order to obtain high cell viabilities combined with06/09/2006· A hydrocyclone with a volume of 256 cm3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume) For the feasible operation range (09 toUse of Hydrocyclones for Mammalian Cell Retention:
Use of Hydrocyclones for Mammalian Cell Retention:
06/09/2006· A hydrocyclone with a volume of 256 cm 3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume) For the feasible operation range (09 to 16 L/min flow corresponding to pressure drops of 04 to 13 bar) the hydrocyclone was characterized with regard to flow split (underflowtooverflow ratio) and flow ratio (underflow toThe results indicate that the hydrocyclone can be appropriately used for cell retention and separation in perfusion cultures A hydrocyclone with a volume of 256 cm3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume) For the feasible operation range (09 to 16 L/min flow corresponding to pressure drops of 04 to 13 bar) theUse of Hydrocyclones for Mammalian Cell Retention: Separation
Hydrocyclones as cell retention device for CHO perfusion
Abstract In this study, a hydrocyclone (HC) especially designed for mammalian cell separation was applied for the separation of Chinese hamster ovary cells The effect of key features on the separation efficiency, such as type of pumphead in the peristaltic feed pump, use of an auxiliary pump to control the perfusate flow rate, and tubing size14/11/2007· The best performance was obtained with a 10mm DorrOliver hydrocyclone, resulting in a separation efficiency of 81% These experimental values agree with computational fluid dynamics (CFD) predictions that high levels of separation efficiencies for mammalian cells could be achieved with smalldiameter hydrocyclones (Medronho et al 2005)Separation of CHO cells using hydrocyclones
Effects of hydrocyclones on the integrity of animal and
The comparative effects of passing mammalian and nucleated red blood cells through liquid/liquid (low shear) and solid/liquid (high shear) hydrocyclones have been examined Cell disruption was only found when the rig was operated in conjunction with a solid/liquid hydrocyclone apparently as a result of the higher operating pressures requiredAbstract In this study, a hydrocyclone (HC) especially designed for mammalian cell separation was applied for the separation of Chinese hamster ovary cells The effect of key features on the separaSkip to Article Content; Skip to Article Information; Search within Search term Advanced Search Citation Search Search term Advanced Search Citation Search Login /Hydrocyclones as cell retention device for CHO perfusion
Separation of CHO cells using hydrocyclones PubMed
When cells were monitored for any induction of apoptosis upon passage through the hydrocyclones, no increase in apoptotic cell concentration was observed within 48 h of hydrocycloning Thus, based on the high separation efficiencies, the robustness of the equipment, and the absence of apoptosis induction, hydrocyclones seem to be specially suited for use14/11/2007· High cell separation efficiencies, varying from 979% to 999%, were obtained for 5 out of 6 hydrocyclone configurations The geometry 3010 (D u = 30 mm and D o = 10 mm) presented a flow ratio of 100%, diverting the total feed flow to the underflow, indicating that this configuration is inappropriate to perform any kind of cell separation Loss of viability wasSeparation of CHO cells using hydrocyclones | SpringerLink
The Use of Hydrocyclones for Mammalian Cell Retention in
separation efficiencies of 81 % for one hydrocyclone and 94% for two in series could be obtained, even employing commercial hydrocyclones that were not specifically designed for application in mammalian cell cultivation (Liibberstedt et aI, 2000a, b; Deckwer et a!, 2000) Moreover, no changes in viability were observed when the hydrocyclones wereAbstract In this study, a hydrocyclone (HC) especially designed for mammalian cell separation was applied for the separation of Chinese hamster ovary cells The effect of key features on the separation efficiency, such as type of pumphead in the peristaltic feed pump, use of an auxiliary pump to control the perfusate flow rate, and tubing sizeHydrocyclones as cell retention device for CHO perfusion
application of hydrocyclone for cell separation in mammalian cell
The present work was carried out to investigate the possibility of hydrocyclone application for cell separation in mammalian cell perfusion cultures Firstly, experiments were carried out to evaluate the physical characteristics controlling the separation performance of the hydrocyclone as well as the effect of the different operating conditions and different hydrocyclone14/11/2007· The best performance was obtained with a 10mm DorrOliver hydrocyclone, resulting in a separation efficiency of 81% These experimental values agree with computational fluid dynamics (CFD) predictions that high levels of separation efficiencies for mammalian cells could be achieved with smalldiameter hydrocyclones (Medronho et al 2005)Separation of CHO cells using hydrocyclones
Application of hydrocyclones for continuous cultivation of SP2/0 cells
22/11/2011· Hydrocyclones (HCs) have been recently extensively evaluated for their application in the separation of mammalian cells in perfusion bioreactors The high centrifugal force derived within hydrocyclones is the key mechanism for cell separation inside such smallsized simple devices This is usually accompanied by a very short residence time ofApplication of Hydrocyclone for Cell Separation in Mammalian Cell Perfusion Cultures Ebook written by Elsayed Ahmed Elsayed Ahmed Read this book using Google Play Books app on your PC, android, iOS devices Download for offline reading, highlight, bookmark or take notes while you read Application of Hydrocyclone for Cell Separation in Mammalian Cell PerfusionApplication of Hydrocyclone for Cell Separation in Mammalian Cell
Data Hydrocyclone SBL2001e Hydrocyclone Mammalian cell
hydrocyclone mammalian cell retention device for and reusable bioreactors perfusion technologies overflow (harvest) feed feed product specification material Anmelden Registrieren Anmelden RegistrierenApplication of Hydrocyclone for Cell Separation in Mammalian Cell Perfusion Cultures Vom Fachbereich für Biowissenschaften und Psychologie der Technischen Universität CaroloWilhelmina zu Braunschweig zur Erlangung des Grades eines Doktors der Naturwissenschaften (Dr rer nat) genehmigte Dissertation von Elsayed Ahmed Elsayed Ahmed aus Kairo,Application of Hydrocyclone for Cell Separation in Mammalian Cell
Acoustic Settler, Centrifuge, Hydrocyclone Animal
Hydrocyclone A detailed description of the hydrocyclone for mammalian cell culture can be found in Castilho's review (Castilho and Medronho 2002) Hydrocyclones are often used to separate solids from liquids or liquids withAlthough DNA breakage and reunion in nonhomologous recombination are poorly understood, previous work suggests that short sequence homologies may play a role in the endjoining step in mammalian cells To study the mechanism of end joining in more detail, we inserted a polylinker into the simian virus 40 Tantigen intron, cleaved the polylinker with different pairs of restrictionNonhomologous recombination in mammalian cells: role for
(PDF) The Potential of Hydrocyclone Application for Mammalian Cell
The Potential of Hydrocyclone Application for Mammalian Cell Separation in Perfusion Cultivation Bioreactors International Journal of Biotechnology for Wellness Industries, 2013The comparative effects of passing mammalian and nucleated red blood cells through liquid/liquid (low shear) and solid/liquid (high shear) hydrocyclones have been examined Cell disruption was only found when the rig was operated in conjunction with a solid/liquid hydrocyclone apparently as a result of the higher operating pressures requiredEffects of hydrocyclones on the integrity of animal and microbial cells
Application of hydrocyclones for continuous cultivation of SP2/0 cells
22/11/2011· Hydrocyclones (HCs) have been recently extensively evaluated for their application in the separation of mammalian cells in perfusion bioreactors The high centrifugal force derived within hydrocyclones is the key mechanism for cell separation inside such smallsized simple devices This is usually accompanied by a very short residence time ofThe present work was carried out to investigate the possibility of hydrocyclone application for cell separation in mammalian cell perfusion cultures Firstly, experiments were carried out to evaluate the physical characteristics controlling the separation performance of the hydrocyclone as well as the effect of the different operating conditions and different hydrocycloneApplication of Hydrocyclone for Cell Separation in Mammalian Cell
hydrocyclone mammalian cells
Use of Hydrocyclones for Mammalian Cell Retention · A hydrocyclone with a volume of 256 cm3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume) For the feasible operation range (09 to 16 L/min flow corresponding to pressure drops of 04 to 13 bar) the hydrocyclone was characterized with regard to flow splitHighly Efficient Extraction of Aromatic Compounds from Houttuyniacordata thumb by Cryogenic Grinding TechniquesAbstract : The Potential of Hydrocyclone Application for Mammalian Cell
A twostep microengineered system for highdensity cell
01/01/2021· The use of hydrocyclones for mammalian cell separation has previously been shown as well , , , However, those studies were limited to low cell densities (<10 million cells/mL) [9] , [45] Few propose that further development of these hydrocyclones, particularly if optimized to specifically suit mammalian cells, could make them competitive withFor the first time, a hydrocyclone specially designed for mammalian cell separation is reported to enable perfusion processes at cell densities in the range of 20 to 50 million/mL for 20 to 25 days Also for the first time, a HC is connected to a singleuse bioreactorHydrocyclone for mAb production in a perfusion singleuse
A Review on Centrifugation in the Pharmaceutical Industry
02/05/2015· Nycodenz®, Histodenz™ Mammalian cells, organelles, membrane vesicles, viruses Iodixanol Mammalian cells, organelles, membrane vesicles, viruses, plasma lipoproteins, proteins, DNA Colloidal silica media Percoll® Mammalian cells, organelles, membrane vesicles (infrequent) 5 Classification of Industrial Centrifuges A Perforated bowl or filter types 1Although DNA breakage and reunion in nonhomologous recombination are poorly understood, previous work suggests that short sequence homologies may play a role in the endjoining step in mammalian cells To study the mechanism of end joining in more detail, we inserted a polylinker into the simian virus 40 Tantigen intron, cleaved the polylinker with different pairs of restrictionNonhomologous recombination in mammalian cells: role for
نتيجة mhada مطاحن تطبيق بجعة lutilisé de Granite de skikda الرمل الاصطناعي عملية التصنيع محطم بنغالور الهند الذهب المحمول ماكينات التعدين جنوب أفريقيا How To Finance A Quarry الاقتصاد في استهلاك الطاقة واستهلاك الحد من ممارسة مركزات النحاس رموز الأجهزة والمعدات الكيميائية pper portable crusher manufacturer in nigeria separation de kumba minerai de Cuivre boue أعمال محطم الحجر cardamom crusher machine کارخانه سنگ شکن شن و ماسه در هند millenium milling and mining عينة من الأعمال شكرا لكم لحضور الحدث crusher first issued fournisseurs de materiel minier afrique du sud مطحنة كهربائية صغيرة محمولة Price Fixed Crusher In Morocco kaolin mines world wide concasseurs chilecomparte تكلفة طحن مطحنة في إثيوبيا application letter format for attend exhibition الناقل، رومانيا تكاليف محطة كسارة تأثير المحمول اتفاق الشركة مع آلة ثقيلة للصخرة محطم في أمريكا تكلفة مصنع معالجة الرمل الاصطناعي للشراء سحق خام الذهب في المنزل لاستخراج المواد كسارة الحجر القصيم الاسمنت ورشة عمل المحجر شركة european impact crusher used in metallurgy with competitive priceQUICK LINKS
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